| 质粒名称: | pBABE-puro SV40 LT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13970 |
| 存储实验室: | Thomas Roberts |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SV40 LT,Simian virus-40 large-T antigen |
| 物种来源: | -- |
| 插入大小: | 2200 bp |
| 基因突变: | -- |
| GenBank编号 | AF316141 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI？（如果销毁则未知) |
| 3' 克隆位点: | BamHI？（如果销毁则未知) |
| 5' 测序引物 | pBABE 5' |
| 3' 测序引物 | pBABE 3' |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene plasmid # 13970 ; http://n2t.net/addgene:13970 ; RRID:Addgene_13970) |
| 参考文献部分: | Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM, Hahn WC. Cancer Cell. 2003 May . 3(5):483-95. 10.1016/S1535-6108(03)00088-6 PubMed 12781366 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE-puro SV40 LT 7273 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABE-puro SV40 LT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7273)
AUTHORS Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM,
Hahn WC
TITLE Human mammary epithelial cell transformation through the activation
of phosphatidylinositol 3-kinase.
JOURNAL Cancer Cell. 2003 May . 3(5):483-95.
PUBMED 12781366
REFERENCE 2 (bases 1 to 7273)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7273
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 12..481
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 546..745
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 946..1362
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1292..1314
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1330..1346
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1400..3526
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
primer_bind complement(3607..3627)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3612..3941
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3792..3927
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3854..3873
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3951..4550
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3951..3970)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4447..4467
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 4771..5240
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5370..5387)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5541..6129)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5621..5640)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6289..7149)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6912..6931
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7150..7254)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ccacctgcag cctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
61 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
121 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
181 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
241 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
301 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg
361 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
421 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
481 atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg
541 gaggcaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat
601 tttatgcgcc tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt
661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg
721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga
781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg
901 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc
961 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag
1021 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt
1081 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc
1141 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg
1201 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg
1261 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct
1321 cgatcctccc tttatccagc cctcactcct tctctaggcg ccggccggat cccgcgaccg
1381 gtcgaccagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
1441 ggaccttcta ggtcttgaaa ggagtgcctg ggggaatatt cctctgatga gaaaggcata
1501 tttaaaaaaa tgcaaggagt ttcatcctga taaaggagga gatgaagaaa aaatgaagaa
1561 aatgaatact ctgtacaaga aaatggaaga tggagtaaaa tatgctcatc aacctgactt
1621 tggaggcttc tgggatgcaa ctgagattcc aacctatgga actgatgaat gggagcagtg
1681 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
1741 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
1801 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
1861 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
1921 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
1981 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
2041 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
2101 tgccttgact agagatccat tttctgttat tgaggaaagt ttgccaggtg ggttaaagga
2161 gcatgatttt aatccagaag aagcagagga aactaaacaa gtgtcctgga agcttgtaac
2221 agagtatgca atggaaacaa aatgtgatga tgtgttgtta ttgcttggga tgtacttgga
2281 atttcagtac agttttgaaa tgtgtttaaa atgtattaaa aaagaacagc ccagccacta
2341 taagtaccat gaaaagcatt atgcaaatgc tgctatattt gctgacagca aaaaccaaaa
2401 aaccatatgc caacaggctg ttgatactgt tttagctaaa aagcgggttg atagcctaca
2461 attaactaga gaacaaatgt taacaaacag atttaatgat cttttggata ggatggatat
2521 aatgtttggt tctacaggct ctgctgacat agaagaatgg atggctggag ttgcttggct
2581 acactgtttg ttgcccaaaa tggattcagt ggtgtatgac tttttaaaat gcatggtgta
2641 caacattcct aaaaaaagat actggctgtt taaaggacca attgatagtg gtaaaactac
2701 attagcagct gctttgcttg aattatgtgg ggggaaagct ttaaatgtta atttgccctt
2761 ggacaggctg aactttgagc taggagtagc tattgaccag tttttagtag tttttgagga
2821 tgtaaagggc actggagggg agtccagaga tttgccttca ggtcagggaa ttaataacct
2881 ggacaattta agggattatt tggatggcag tgttaaggta aacttagaaa agaaacacct
2941 aaataaaaga actcaaatat ttccccctgg aatagtcacc atgaatgagt acagtgtgcc
3001 taaaacactg caggccagat ttgtaaaaca aatagatttt aggcccaaag attatttaaa
3061 gcattgcctg gaacgcagtg agtttttgtt agaaaagaga ataattcaaa gtggcattgc
3121 tttgcttctt atgttaattt ggtacagacc tgtggctgag tttgctcaaa gtattcagag
3181 cagaattgtg gagtggaaag agagattgga caaagagttt agtttgtcag tgtatcaaaa
3241 aatgaagttt aatgtggcta tgggaattgg agttttagat tggctaagaa acagtgatga
3301 tgatgatgaa gacagccagg aaaatgctga taaaaatgaa gatggtgggg agaagaacat
3361 ggaagactca gggcatgaaa caggcattga ttcacagtcc caaggctcat ttcaggcccc
3421 tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac
3481 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
3541 ttgttgttaa cggggatccc agtgtggtgg tacgtaggaa ttcgccagca cagtggtcga
3601 ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
3661 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
3721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
3781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
3841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
3901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt
3961 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
4021 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
4081 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
4141 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
4201 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
4261 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
4321 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
4381 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
4441 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
4501 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
4561 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga
4621 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
4681 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
4741 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
4801 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
4861 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
4921 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
4981 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
5041 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
5101 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
5161 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
5221 ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta
5281 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc
5341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
5401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
5461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
5521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
5581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
5641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
5701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
5761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
5821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
5881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
5941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
6241 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
6301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
6361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
6421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
6481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
6541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
6601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
6661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
6721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
6781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
6841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
6901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
6961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
7021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
7081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
7141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
7261 tccccgaaaa gtg
//
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